Quantification methods for carbohydrate compounds in biologicals: a review

Carbohydrate compounds are widely used as fillers and stabilisers in biological products. When present, these guarantee that the active pharmaceutical ingredient will remain stable during production, transportation, storage. At same time, pharmacopoeias standardise excipient content require excipients should be quantified for assessing quality of The aim study was to identify promising methods development quantification procedures carbohydrate authors analysed regulatory documents products approved Russian Federation. most excipients, both individually combinations, polyols (sorbitol mannitol), monosaccarides (glucose), disaccharides (trehalose, sucrose, lactose, maltose). Using literature data, reviewed quantifying polyols, monosaccharides, assess Quantitative determination employs titrimetric, spectrophotometric, enzymatic, chromatographic methods. This review presents an analysis advantages disadvantages It highlights ionic HPLC with amperometric detection hydrophilic refractometric evaporative light scattering detection, which sufficiently selective can substances without prior derivatisation. In conclusion, a base stabilisers.